8/17/2023 0 Comments Bio rad chromatography column![]() ![]() Microtitration plate reader Tecan Sunrise This system is used for the protein purification steps that do not require high operation pressure (ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography). The system is not PC controlled but together with PrimeView™ software it offers continuous real-time monitoring and evaluation of results. The unit is compact and comprises of a system pump, fraction collector, monitors for UV, conductivity and pH. ÄKTAprime™ plus is a rather simple and user-friendly FPLC for protein chromatography, employing maximum pressure of 1 MPa. This instrument is used for the high performance purification of fungal glycosidases and nitrilases (ion exchange chromatography, hydrophobic interaction chromatography, gel filtration, chromatofocusing, affinity chromatography and reversed phase chromatography). The system is connected to a PC and controlled by Unicorn 5.11 software. Our instrument is equipped with a P-903 pump (2 pump modules A and B, including two pump heads each), Monitor UV-900 (able to detect up to three wavelengths simultaneously), Monitor pH/C-900 (measurement of pH and conductivity, pH electrode), and a Frac-920 fraction collector. This FPLC can operate at maximum flow rate of 10 ml/min with a maximum pressure of 25 MPa, which is sufficient for preparative protein purification on a milligram scale. ÄKTApurifier™ is a versatile protein and peptide purification system compatible with any type of chromatographic columns and solvents. Manufactured by Amersham Biosciences, Sweden 10 MPaĪgilent 6890N network GC system is equipped with single FID, single split/splitless capillary with EPC, G2613A 7683 injector-autosampler, Agilent Chemstation with G2070A single GC software. This up-to-date protein chromatography system is suitable for all types of manually performed or automated protein purification techniques, including affinity, ion-exchange, and hydrophobic chromatography as well as high-pressure gel filtration. NGC QuestTM10 Plus Protein Chromatography System Bio-Rad Laboratories, Inc. Columns available: include normal (silicagel) and RP-18 50×250 mm columns and Asahipack GS-310 multimode GPC column. Preparative fraction collector FRC-10A is controlled by operator or by signal from SPD-20A UV detector. Isocratic preparative HPLC pump LC-8A with mobile phase flow up to 150 ml/min is equipped with manual injection valve Rheodyne. The columns available involve various reversed-phase, normal phase, ion exchange and chiral columns. ![]() The system is equipped with Waters 996 PDA Detector and Waters 2410 Differential Refractometer. The system enables manual or automatic injection by Rheodyne injection valve or Waters717plus Autosampler, respectively. The ternary gradient pump, Waters 600 E Multisolvent Delivery System, provides flows up to 10 ml/min. Two high-pressure solvent delivery units (the LC-20AB), auto-sampler (SIL-20A) column oven (CTO-20A), PDA Detector (SPD-M20A). Auto-sampler (SIL-20AC), column oven (CTO-10A, + 10☌ to 85☌), PDA detector (SPD-M20A) offer a very low baseline noise of less than 0.35×10 –5 AU, fraction collector (FRC-10A), mass detector (LCMS-2020) is a single quadrupole mass spectrometer with ESI ionization. The pumps are configured as high resolution (0.1%) binary- high pressure gradient system. Two high-pressure solvent delivery units (the LC-20AD). Prominence Shimadzu HPLC-LCMS 2020 Configuration Microtitration plate reader Tecan Sunrise.Agilent 6890N Network Gas Chromatograph.NGC QuestTM10 Plus Protein Chromatography System. ![]()
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